International Superstar Soccer 2000 is the second game in the Nintendo 64 Perfect Striker series and the last in Konami's Jikkyo Soccer series of N64 games developed by Konami Computer Entertainment Osaka.

Introduced in the North American and European versions, it was the first and only game in the series to support the high resolution mode through an expansion pack; however, it suffered from some performance issues, affecting the frame rate of the game.

ISS 2000 is also notable for being the only game in the series which included players with real names (such as Rafael Márquez), though these players only appeared in the North American release, and no team had a 100% real roster.