Duke Nukem 64 is the Nintendo 64 port of the first-person shooter MS-DOS/PC game Duke Nukem 3D.

The Nintendo 64 port features significant changes from the PC version, including modified and expanded levels and a different set of weapons. The port also includes a four-player deathmatch mode and a two-player co-op mode via split-screen. The game's mature themes have been minimized to satisfy Nintendo's adult content standards.