Mario & Luigi: Partners in Time is a role-playing video game developed by AlphaDream and published by Nintendo for the Nintendo DS handheld game console in 2005.

The gameplay of Partners in Time, with an emphasis on role-playing game elements and co-operative puzzle-solving, is similar to that of its predecessor, although gameplay differences exist between the games. For the majority of Partners in Time, the overworld of the game is presented on the DS's touchscreen, while a map is present on the top screen, showing relevant information such as the location of each character and save albums. Additionally, the perspective changes when battling or accessing the inventory, and when the quartet is separated into two pairs, in which a pair is visible on each screen.