Professor Layton and the Miracle Mask is a puzzle adventure video game developed by Level-5. It is the fifth game in the Professor Layton series, the second in the prequel trilogy of games, and the first in the series to be developed for the Nintendo 3DS.

The game centers on Professor Hershel Layton, alongside his apprentice, Luke Triton, and his assistant, Emmy Altava, attempting to uncover to discover the truth behind the "Masked Gentleman", a villain who is seemingly terrorising the city of Monte d'Or with supposed uses of "dark magic".

Like previous games in the series, Professor Layton and the Miracle Mask is a puzzle adventure game split across various chapters. Players explore areas and converse with characters to help solve the mysteries that lay before them. At various points in the game, the player will encounter puzzles that need to be solved. Solving these puzzles earn Picarats, used for accessing bonus content, with more Picarats earned for solving the puzzle without any mistakes. Hint coins can also be found which can be spent to unlock hints to help players solve a puzzle if they get stuck.